Overexpression of sucrose phosphate synthase increases sucrose unloading in transformed tomato fruit
نویسندگان
چکیده
منابع مشابه
Antisense inhibition of tomato fruit sucrose synthase decreases fruit setting and the sucrose unloading capacity of young fruit.
The role of sucrose synthase (SuSy) in tomato fruit was studied in transgenic tomato (Lycopersicon esculentum) plants expressing an antisense fragment of fruit-specific SuSy RNA (TOMSSF) under the control of the cauliflower mosaic virus 35S promoter. Constitutive expression of the antisense RNA markedly inhibited SuSy activity in flowers and fruit pericarp tissues. However, inhibition was only ...
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Phloem unloading plays a pivotal role in photoassimilate partitioning and the accumulation of sugars in sink organs, e.g. fruit. Here, we investigated the pathway of sucrose unloading in kiwifruit (Actinidia deliciasa cv. Qinmei) using a combination of electron microscopy, transport of the phloem-mobile symplastic tracer carboxyfluorescein and enzyme activity and gene expression assays. Our str...
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Suc synthase (EC 2. 4. 1. 13) catalyzes the reversible reaction: Suc + UDP c-, UDP-Glc + Fru. In plants, its main physiological function is to cleave Suc and generate UDPGlc, which is used in such diverse pathways as starch synthesis, cell wall component synthesis, and energy generation (Huber and Akazawa, 1986; Sung et al., 1988). It has been demonstrated that Suc synthase is a key enzyme duri...
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Contrasting evidence has accumulated regarding the role of acid invertase and sucrose synthase in tomato fruit sink establishment and maintenance. In this work the relationships among the activities of sucrose synthase and acid invertase, Lycopersicon esculentum Mill cv UC-82B fruit growth, and starch accumulation were analyzed in fruit at 0 to 39 d after anthesis. Sucrose synthase, but not aci...
متن کاملSucrose phosphate synthase and sucrose accumulation at low temperature.
The influence of growth temperature on the free sugar and sucrose phosphate synthase content and activity of spinach (Spinacia oleracea) leaf tissue was studied. When plants were grown at 25 degrees C for 3 weeks and then transferred to a constant 5 degrees C, sucrose, glucose, and fructose accumulated to high levels during a 14-d period. Predawn sugar levels increased from 14- to 20-fold over ...
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 1999
ISSN: 0022-0957,1460-2431
DOI: 10.1093/jxb/50.335.785